Analytical Technologies Singapore

The pco.flim camera system is the first luminescence decay time microscopy camera using a two-tap CMOS image sensor. Synchronized modulation of pixels and stimulated light enables the analysis of decay times in the range of 100 ps to 100 µs. A wide variety of trigger options allows the camera’s integration in versatile fields of applications.

The pco.flim laser is an excitation and illumination light source designed for use with the FD-FLIM pco.flim camera system. It features a digital (square wave) modulation frequency range of 0 – 250 MHz, which ideally covers the modulation frequency range of 5 kHz – 40 MHz offered by the pco.flim camera.

There is a full range of different wavelengths available (375 nm – 395 nm – 405 nm – 445 nm – 473 nm – 488 nm – 515 nm – 638 nm). The system is optimum suited for the measurement of fluorescence lifetimes in the range of nanoseconds.

  • pco.flim specification
  • pco.FLIM Laser

Image Sensor

Type of sensorCMOS
Image sensorproprietary
Resolution (h x v)1008 x 1008 pixels
Pixel size (h x v)5.6 µm x 5.6 µm
Sensor format / diagonal5.7 mm x 5.7 mm / 8.1 mm
Shutter moderolling reset / global exposure
Fullwell capacity52 000 e- (typ.)
Readout Noise48 e- rms (typ.)
Dynamic Range> 1 000 : 1 (60 dB)
Quantum Efficiencyappr. 39 % @ peak
Spectral Range370 nm ... 780 nm (FWHM)
Dark Current1220 e- / (s.pixel)
DSNU56 e- rms
PRNU0.7 %

 

Wavelengths & Powers (selectable; other wavelengths and powers on request)• 375 nm / 70 mW
• 395 nm / 120 mW
• 405 nm / 120 mW
• 445 nm / 100 mW
• 473 nm / 100 mW
• 488 nm / 100 mW
• 515 nm / 100 mW
• 638 nm / 150 mW
Opto-mechanical interface
(selectable)
• 3 mm liquid light guide
• FC/APC
• FC/PC
• FCP8
• 0.7 mm collimated beam
Long Term Power Stability< 0.5 % / 8 h
RMS noise20 Hz .. 20 MHz < 0.2 % (CW)
Analogue modulation
input signal type
3 MHz (0.5 V @ 1.2 kOhm\r\nor 0..1 V @ 50 Ohm - user configurable"}">> 3 MHz (0.5 V @ 1.2 kOhm
or 0..1 V @ 50 Ohm - user configurable
Digital Modulation
input signal type
250 MHz ( +/- 0.5 V @ 50 Ohm)"}">> 250 MHz ( +/- 0.5 V @ 50 Ohm)
Laser enable (electronic shutter)
input signal type
500 kHz\r\n(full ON/OFF) (LV-TTL @ 2 kOhm)"}">> 500 kHz
(full ON/OFF) (LV-TTL @ 2 kOhm)
Operation Modes:
Mode 1CW operation
(ACC - automatic constant current)
Mode 2CW operation
(ACC - automatic power control)
Mode 3Analogue Modulation
Mode 4Digital modulation
Mode 5Analogue + Digital modulation
Rise and Fall timeanalogue < 100 ns
digital < 1.5 ns
laser enable < 100 ns
Extinction Ratio1000 : 1\ndigital > 250 : 1\nlaser enable: infinite (full ON/OFF)"}">analogue > 1000 : 1
digital > 250 : 1
laser enable: infinite (full ON/OFF)
Supply Voltage12.0 VDC +/- 0.5 VDC
Control Interface USB 2.0USB 2.0
Dimensions (L x W x H)270 mm x 92 mm x 116 mm
Additional ShutterOptional: Nikon laser safety shutter

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